Ultrastructure of plant leaf cuticles in relation to sample preparation as observed by transmission electron microscopy.
Identifieur interne : 001F80 ( Main/Exploration ); précédent : 001F79; suivant : 001F81Ultrastructure of plant leaf cuticles in relation to sample preparation as observed by transmission electron microscopy.
Auteurs : Paula Guzmán [Espagne] ; Victoria Fernández [Espagne] ; Mohamed Khayet [Espagne] ; María Luisa García [Espagne] ; Agustín Fernández [Espagne] ; Luis Gil [Espagne]Source :
- TheScientificWorldJournal [ 1537-744X ] ; 2014.
Descripteurs français
- KwdFr :
- MESH :
- méthodes : Microscopie électronique à transmission.
- ultrastructure : Feuilles de plante, Populus, Pyrus, Épiderme végétal.
English descriptors
- KwdEn :
- MESH :
- methods : Microscopy, Electron, Transmission.
- ultrastructure : Plant Epidermis, Plant Leaves, Populus, Pyrus.
Abstract
The leaf cuticular ultrastructure of some plant species has been examined by transmission electron microscopy (TEM) in only few studies. Attending to the different cuticle layers and inner structure, plant cuticles have been grouped into six general morphological types. With the aim of critically examining the effect of cuticle isolation and preparation for TEM analysis on cuticular ultrastructure, adaxial leaf cuticles of blue-gum eucalypt, grey poplar, and European pear were assessed, following a membrane science approach. The embedding and staining protocols affected the ultrastructure of the cuticles analysed. The solubility parameter, surface tension, and contact angles with water of pure Spurr's and LR-White resins were within a similar range. Differences were however estimated for resin : solvent mixtures, since Spurr's resin is combined with acetone and LR-White resin is mixed with ethanol. Given the composite hydrophilic and lipophilic nature of plant cuticles, the particular TEM tissue embedding and staining procedures employed may affect sample ultrastructure and the interpretation of the results in physicochemical and biological terms. It is concluded that tissue preparation procedures may be optimised to facilitate the observation of the micro- and nanostructure of cuticular layers and components with different degrees of polarity and hydrophobicity.
DOI: 10.1155/2014/963921
PubMed: 24895682
PubMed Central: PMC4032664
Affiliations:
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transmission electron microscopy.</title><author><name sortKey="Guzman, Paula" sort="Guzman, Paula" uniqKey="Guzman P" first="Paula" last="Guzmán">Paula Guzmán</name><affiliation wicri:level="2"><nlm:affiliation>Forest Genetics and Ecophysiology Research Group, School of Forest Engineering, Technical University of Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Forest Genetics and Ecophysiology Research Group, School of Forest Engineering, Technical University of Madrid, Ciudad Universitaria s/n, 28040 Madrid</wicri:regionArea><placeName><region nuts="2" type="communauté">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Fernandez, Victoria" sort="Fernandez, Victoria" uniqKey="Fernandez V" first="Victoria" last="Fernández">Victoria Fernández</name><affiliation wicri:level="2"><nlm:affiliation>Forest Genetics and Ecophysiology Research Group, School of Forest Engineering, Technical University of Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Forest Genetics and Ecophysiology Research Group, School of Forest Engineering, Technical University of Madrid, Ciudad Universitaria s/n, 28040 Madrid</wicri:regionArea><placeName><region nuts="2" type="communauté">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Khayet, Mohamed" sort="Khayet, Mohamed" uniqKey="Khayet M" first="Mohamed" last="Khayet">Mohamed Khayet</name><affiliation wicri:level="2"><nlm:affiliation>Department of Applied Physics I, Faculty of Physics, University Complutense of Madrid, Avenida Complutense s/n, 28040 Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Applied Physics I, Faculty of Physics, University Complutense of Madrid, Avenida Complutense s/n, 28040 Madrid</wicri:regionArea><placeName><region nuts="2" type="communauté">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Garcia, Maria Luisa" sort="Garcia, Maria Luisa" uniqKey="Garcia M" first="María Luisa" last="García">María Luisa García</name><affiliation wicri:level="2"><nlm:affiliation>Electron Microscopy National Centre, Faculty of Chemistry, University Complutense of Madrid, Avenida Complutense s/n, 28040 Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Electron Microscopy National Centre, Faculty of Chemistry, University Complutense of Madrid, Avenida Complutense s/n, 28040 Madrid</wicri:regionArea><placeName><region nuts="2" type="communauté">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Fernandez, Agustin" sort="Fernandez, Agustin" uniqKey="Fernandez A" first="Agustín" last="Fernández">Agustín Fernández</name><affiliation wicri:level="2"><nlm:affiliation>Electron Microscopy National Centre, Faculty of Chemistry, University Complutense of Madrid, Avenida Complutense s/n, 28040 Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Electron Microscopy National Centre, Faculty of Chemistry, University Complutense of Madrid, Avenida Complutense s/n, 28040 Madrid</wicri:regionArea><placeName><region nuts="2" type="communauté">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Gil, Luis" sort="Gil, Luis" uniqKey="Gil L" first="Luis" last="Gil">Luis Gil</name><affiliation wicri:level="2"><nlm:affiliation>Forest Genetics and Ecophysiology Research Group, School of Forest Engineering, Technical University of Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Forest Genetics and Ecophysiology Research Group, School of Forest Engineering, Technical University of Madrid, Ciudad Universitaria s/n, 28040 Madrid</wicri:regionArea><placeName><region nuts="2" type="communauté">Communauté de Madrid</region></placeName></affiliation></author></analytic><series><title level="j">TheScientificWorldJournal</title><idno type="eISSN">1537-744X</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Microscopy, Electron, Transmission (methods)</term><term>Plant Epidermis (ultrastructure)</term><term>Plant Leaves (ultrastructure)</term><term>Populus (ultrastructure)</term><term>Pyrus (ultrastructure)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Feuilles de plante (ultrastructure)</term><term>Microscopie électronique à transmission (méthodes)</term><term>Populus (ultrastructure)</term><term>Pyrus (ultrastructure)</term><term>Épiderme végétal (ultrastructure)</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Microscopy, Electron, Transmission</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Microscopie électronique à transmission</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Plant Epidermis</term><term>Plant Leaves</term><term>Populus</term><term>Pyrus</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="fr"><term>Feuilles de plante</term><term>Populus</term><term>Pyrus</term><term>Épiderme végétal</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The leaf cuticular ultrastructure of some plant species has been examined by transmission electron microscopy (TEM) in only few studies. Attending to the different cuticle layers and inner structure, plant cuticles have been grouped into six general morphological types. With the aim of critically examining the effect of cuticle isolation and preparation for TEM analysis on cuticular ultrastructure, adaxial leaf cuticles of blue-gum eucalypt, grey poplar, and European pear were assessed, following a membrane science approach. The embedding and staining protocols affected the ultrastructure of the cuticles analysed. The solubility parameter, surface tension, and contact angles with water of pure Spurr's and LR-White resins were within a similar range. Differences were however estimated for resin : solvent mixtures, since Spurr's resin is combined with acetone and LR-White resin is mixed with ethanol. Given the composite hydrophilic and lipophilic nature of plant cuticles, the particular TEM tissue embedding and staining procedures employed may affect sample ultrastructure and the interpretation of the results in physicochemical and biological terms. It is concluded that tissue preparation procedures may be optimised to facilitate the observation of the micro- and nanostructure of cuticular layers and components with different degrees of polarity and hydrophobicity.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24895682</PMID><DateCompleted><Year>2015</Year><Month>01</Month><Day>12</Day></DateCompleted><DateRevised><Year>2019</Year><Month>01</Month><Day>12</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1537-744X</ISSN><JournalIssue CitedMedium="Internet"><Volume>2014</Volume><PubDate><Year>2014</Year></PubDate></JournalIssue><Title>TheScientificWorldJournal</Title><ISOAbbreviation>ScientificWorldJournal</ISOAbbreviation></Journal><ArticleTitle>Ultrastructure of plant leaf cuticles in relation to sample preparation as observed by transmission electron microscopy.</ArticleTitle><Pagination><MedlinePgn>963921</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1155/2014/963921</ELocationID><Abstract><AbstractText>The leaf cuticular ultrastructure of some plant species has been examined by transmission electron microscopy (TEM) in only few studies. Attending to the different cuticle layers and inner structure, plant cuticles have been grouped into six general morphological types. With the aim of critically examining the effect of cuticle isolation and preparation for TEM analysis on cuticular ultrastructure, adaxial leaf cuticles of blue-gum eucalypt, grey poplar, and European pear were assessed, following a membrane science approach. The embedding and staining protocols affected the ultrastructure of the cuticles analysed. The solubility parameter, surface tension, and contact angles with water of pure Spurr's and LR-White resins were within a similar range. Differences were however estimated for resin : solvent mixtures, since Spurr's resin is combined with acetone and LR-White resin is mixed with ethanol. Given the composite hydrophilic and lipophilic nature of plant cuticles, the particular TEM tissue embedding and staining procedures employed may affect sample ultrastructure and the interpretation of the results in physicochemical and biological terms. It is concluded that tissue preparation procedures may be optimised to facilitate the observation of the micro- and nanostructure of cuticular layers and components with different degrees of polarity and hydrophobicity.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Guzmán</LastName><ForeName>Paula</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Forest Genetics and Ecophysiology Research Group, School of Forest Engineering, Technical University of Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fernández</LastName><ForeName>Victoria</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>Forest Genetics and Ecophysiology Research Group, School of Forest Engineering, Technical University of Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Khayet</LastName><ForeName>Mohamed</ForeName><Initials>M</Initials><Identifier Source="ORCID">0000-0002-5117-2975</Identifier><AffiliationInfo><Affiliation>Department of Applied Physics I, Faculty of Physics, University Complutense of Madrid, Avenida Complutense s/n, 28040 Madrid, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>García</LastName><ForeName>María Luisa</ForeName><Initials>ML</Initials><AffiliationInfo><Affiliation>Electron Microscopy National Centre, Faculty of Chemistry, University Complutense of Madrid, Avenida Complutense s/n, 28040 Madrid, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fernández</LastName><ForeName>Agustín</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Electron Microscopy National Centre, Faculty of Chemistry, University Complutense of Madrid, Avenida Complutense s/n, 28040 Madrid, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gil</LastName><ForeName>Luis</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Forest Genetics and Ecophysiology Research Group, School of Forest Engineering, Technical University of Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>04</Month><Day>22</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>ScientificWorldJournal</MedlineTA><NlmUniqueID>101131163</NlmUniqueID><ISSNLinking>1537-744X</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D046529" MajorTopicYN="N">Microscopy, Electron, Transmission</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019441" MajorTopicYN="N">Plant Epidermis</DescriptorName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000648" MajorTopicYN="Y">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D031989" MajorTopicYN="N">Pyrus</DescriptorName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2014</Year><Month>02</Month><Day>06</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2014</Year><Month>03</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>6</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>6</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>1</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24895682</ArticleId><ArticleId IdType="doi">10.1155/2014/963921</ArticleId><ArticleId IdType="pmc">PMC4032664</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Front Microbiol. 2011 Sep 29;2:197</Citation><ArticleIdList><ArticleId IdType="pubmed">22046169</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol Biochem. 2014 Mar;76:1-6</Citation><ArticleIdList><ArticleId 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